iTunes backup password is required each time I run analysis
Normally when you extract iTunes backup and a temporary password is set-up by MOBILedit Forensic Express is this password removed after successful extraction.
It looks like your first extraction didn't finish successfully and the iTunes Backup remained encrypted with temporary password, for such cases there is an option to remove the password when you connect the device.
The iTunes backup might also be encrypted prior to the extraction, therefore you will be asked to provide the password. In case it is unknown to you, there is an option to use our password breaker to brute-force through it. Find more info on Password breaker here!